In Vitro Antioxidant and Antiproliferative Activities of Novel Orange Peel Extract and It's Fractions on Leukemia HL-60 Cells.
Identifieur interne : 000585 ( Main/Exploration ); précédent : 000584; suivant : 000586In Vitro Antioxidant and Antiproliferative Activities of Novel Orange Peel Extract and It's Fractions on Leukemia HL-60 Cells.
Auteurs : Kawthar A E. Diab [Oman] ; Reham Ezzat Shafik ; Shin YasudaSource :
- Asian Pacific journal of cancer prevention : APJCP [ 1513-7368 ] ; 2015.
English descriptors
- KwdEn :
- Cell Proliferation (drug effects), Cell Survival (drug effects), Citrus sinensis, DNA Fragmentation (drug effects), Dose-Response Relationship, Drug, Flavonoids (analysis), Free Radical Scavengers (pharmacology), Fruit, HL-60 Cells, Humans, Inhibitory Concentration 50, Plant Extracts (chemistry), Plant Extracts (pharmacology), Polyphenols (analysis).
- MESH :
- chemical , analysis : Flavonoids, Polyphenols.
- chemical , chemistry : Plant Extracts.
- drug effects : Cell Proliferation, Cell Survival, DNA Fragmentation.
- chemical , pharmacology : Free Radical Scavengers, Plant Extracts.
- Citrus sinensis, Dose-Response Relationship, Drug, Fruit, HL-60 Cells, Humans, Inhibitory Concentration 50.
Abstract
In the present work, novel orange peel was extracted with 100%EtOH (ethanol) and fractionated into four fractions namely F1, F2, F3, F4 which were eluted from paper chromatographs using 100%EtOH, 80%EtOH, 50%EtOH and pure water respectively. The crude extract and its four fractions were evaluated for their total polyphenol content (TPC), total flavonoid content (TFC) and radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Their cytotoxic activity using WST assay and DNA damage by agarose gel electrophoresis were also evaluated in a human leukemia HL-60 cell line. The findings revealed that F4 had the highest TPC followed by crude extract, F2, F3 and F1. However, the crude extract had the highest TFC followed by F4, F3, F2, and F1. Depending on the values of EC50 and trolox equivalent antioxidant capacity, F4 possessed the strongest antioxidant activity while F1 and F2 displayed weak antioxidant activity. Further, incubation HL-60 cells with extract/fractions for 24h caused an inhibition of cell viability in a concentration- dependent manner. F3 and F4 exhibited a high antiproliferative activity with a narrow range of IC50 values (45.9 - 48.9 μg/ml). Crude extract exhibited the weakest antiproliferative activity with an IC50 value of 314.89 μg/ml. Analysis of DNA fragmentation displayed DNA degradation in the form of a smear-type pattern upon agarose gel after incubation of HL-60 cells with F3 and F4 for 6 h. Overall, F3 and F4 appear to be good sources of phytochemicals with antioxidant and potential anticancer activities.
PubMed: 26514490
Affiliations:
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Diab</name><affiliation wicri:level="1"><nlm:affiliation>Genetics and Cytology Department, National Research Centre, Cairo, Egypt E-mail : kawthar_diab@yahoo.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Genetics and Cytology Department, National Research Centre, Cairo</wicri:regionArea><wicri:noRegion>Cairo</wicri:noRegion></affiliation></author><author><name sortKey="Shafik, Reham Ezzat" sort="Shafik, Reham Ezzat" uniqKey="Shafik R" first="Reham Ezzat" last="Shafik">Reham Ezzat Shafik</name></author><author><name sortKey="Yasuda, Shin" sort="Yasuda, Shin" uniqKey="Yasuda S" first="Shin" last="Yasuda">Shin Yasuda</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26514490</idno><idno type="pmid">26514490</idno><idno type="wicri:Area/PubMed/Corpus">000238</idno><idno type="wicri:Area/PubMed/Curation">000238</idno><idno type="wicri:Area/PubMed/Checkpoint">000238</idno><idno type="wicri:Area/Ncbi/Merge">001D77</idno><idno type="wicri:Area/Ncbi/Curation">001D77</idno><idno type="wicri:Area/Ncbi/Checkpoint">001D77</idno><idno type="wicri:doubleKey">1513-7368:2015:Diab K:in:vitro:antioxidant</idno><idno type="wicri:Area/Main/Merge">000585</idno><idno type="wicri:Area/Main/Curation">000585</idno><idno type="wicri:Area/Main/Exploration">000585</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">In Vitro Antioxidant and Antiproliferative Activities of Novel Orange Peel Extract and It's Fractions on Leukemia HL-60 Cells.</title><author><name sortKey="Diab, Kawthar A E" sort="Diab, Kawthar A E" uniqKey="Diab K" first="Kawthar A E" last="Diab">Kawthar A E. Diab</name><affiliation wicri:level="1"><nlm:affiliation>Genetics and Cytology Department, National Research Centre, Cairo, Egypt E-mail : kawthar_diab@yahoo.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Genetics and Cytology Department, National Research Centre, Cairo</wicri:regionArea><wicri:noRegion>Cairo</wicri:noRegion></affiliation></author><author><name sortKey="Shafik, Reham Ezzat" sort="Shafik, Reham Ezzat" uniqKey="Shafik R" first="Reham Ezzat" last="Shafik">Reham Ezzat Shafik</name></author><author><name sortKey="Yasuda, Shin" sort="Yasuda, Shin" uniqKey="Yasuda S" first="Shin" last="Yasuda">Shin Yasuda</name></author></analytic><series><title level="j">Asian Pacific journal of cancer prevention : APJCP</title><idno type="ISSN">1513-7368</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Proliferation (drug effects)</term><term>Cell Survival (drug effects)</term><term>Citrus sinensis</term><term>DNA Fragmentation (drug effects)</term><term>Dose-Response Relationship, Drug</term><term>Flavonoids (analysis)</term><term>Free Radical Scavengers (pharmacology)</term><term>Fruit</term><term>HL-60 Cells</term><term>Humans</term><term>Inhibitory Concentration 50</term><term>Plant Extracts (chemistry)</term><term>Plant Extracts (pharmacology)</term><term>Polyphenols (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Flavonoids</term><term>Polyphenols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Proliferation</term><term>Cell Survival</term><term>DNA Fragmentation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Free Radical Scavengers</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Citrus sinensis</term><term>Dose-Response Relationship, Drug</term><term>Fruit</term><term>HL-60 Cells</term><term>Humans</term><term>Inhibitory Concentration 50</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the present work, novel orange peel was extracted with 100%EtOH (ethanol) and fractionated into four fractions namely F1, F2, F3, F4 which were eluted from paper chromatographs using 100%EtOH, 80%EtOH, 50%EtOH and pure water respectively. The crude extract and its four fractions were evaluated for their total polyphenol content (TPC), total flavonoid content (TFC) and radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Their cytotoxic activity using WST assay and DNA damage by agarose gel electrophoresis were also evaluated in a human leukemia HL-60 cell line. The findings revealed that F4 had the highest TPC followed by crude extract, F2, F3 and F1. However, the crude extract had the highest TFC followed by F4, F3, F2, and F1. Depending on the values of EC50 and trolox equivalent antioxidant capacity, F4 possessed the strongest antioxidant activity while F1 and F2 displayed weak antioxidant activity. Further, incubation HL-60 cells with extract/fractions for 24h caused an inhibition of cell viability in a concentration- dependent manner. F3 and F4 exhibited a high antiproliferative activity with a narrow range of IC50 values (45.9 - 48.9 μg/ml). Crude extract exhibited the weakest antiproliferative activity with an IC50 value of 314.89 μg/ml. Analysis of DNA fragmentation displayed DNA degradation in the form of a smear-type pattern upon agarose gel after incubation of HL-60 cells with F3 and F4 for 6 h. Overall, F3 and F4 appear to be good sources of phytochemicals with antioxidant and potential anticancer activities.</div></front></TEI><affiliations><list><country><li>Oman</li></country></list><tree><noCountry><name sortKey="Shafik, Reham Ezzat" sort="Shafik, Reham Ezzat" uniqKey="Shafik R" first="Reham Ezzat" last="Shafik">Reham Ezzat Shafik</name><name sortKey="Yasuda, Shin" sort="Yasuda, Shin" uniqKey="Yasuda S" first="Shin" last="Yasuda">Shin Yasuda</name></noCountry><country name="Oman"><noRegion><name sortKey="Diab, Kawthar A E" sort="Diab, Kawthar A E" uniqKey="Diab K" first="Kawthar A E" last="Diab">Kawthar A E. 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